YB90584Mu01

Heme Oxygenase 1, Decycling (HO1)

Organism: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

3th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Heme Oxygenase 1, Decycling

Synonyms: HO1

Species: Mouse

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Microsome. Endoplasmic reticulum.

[ PROPERTIES ]

Residues: Pro99~Arg237 (Accession # P14901), with two N-terminal Tags, His-tag and

S-tag.

Grade & Purity: >97%,21.6 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 21.6 kDa

Predicted isoelectric point: 6.26

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with two N-terminal tags, His-tag and S-tag, its sequence is listed below.

MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-PH WQEIIPCTPA

TQHYVKRLHE VGRTHPELLV AHAYTRYLGD LSGGQVLKKI AQKAMALPSS GEGLAFFTFP NIDSPTKFKQ

LYRARMNTLE MTPEVKHRVT EEAKTAFLLN IELFEELQVM LTEEHKDQSP SQMASLR

[ REFERENCES ]

1.Yachie A., et.al. (1999). J Clin Invest 103 (1): 129–35.

2.Wang J., et.al. (2007). Brain 130 (6): 1643–52.

3.Soares MP., et.al. (2002). Immunol. Rev. 184: 275–85.

4.Morse D., et.al. (2002). Am. J. Respir. Cell Mol. Biol. 27 (1): 8–16.

5.Buelow R., et.al. (2002). Am. J. Transplant. 1 (4): 313–5.

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