YB80838Ra01

Otubain 1 (OTUB1)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Otubain 1

Gene Names: OTUB1

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination. Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.

Subcellular Location: Cytoplasm

[ PROPERTIES ]

Residues: Met1~Lys271 (Accession # B2RYG6), with a N-terminal His-tag.

Grade & Purity: >97%, 32.51 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 32.51 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

MGHHHHHHSGS-MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE

YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH  LEALLDDSKE  LQRFKAVSAK SKEDLVSQGF

TEFTIEDFHN  TFMDLIEQVE KQTSVADLLA  SFNDQSTSDY  LVVYLRLLTS  GYLQRESKFF EHFIEGGRTV

KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K

[ REFERENCES ]

1.Maurya D.K., et al. (2009) Proteomics. 9:2593-2599.

2.Strausberg RL, et al. (2002) Proc Natl Acad Sci U S A.99(26):16899-16903.

3.The MGC Project Team. (2004) Genome Res. 14:2121-2127

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