YB90141Ra01

Plasminogen Activator, Urokinase Receptor (uPAR)

Organism:Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Rat uPAR  kDa Protein Names: Plasminogen Activator, Urokinase Receptor

Synonyms: U-PAR, uPAR

Species: Rat

Size: 100 µg

Source: Escherichia coli -derived

Subcellular Location: Isoform 1: cell membrane; Lipid-anchor; GPI-anchor; Isoform

2:secreted.

[ PROPERTIES ]

Residues: Thr17~Asn212 (Accession # P49616), with one N-terminal His Tag.

Grade & Purity: >95%, 25 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in Phosphate buffered saline(PBS), pH 7.4，

containing 0.01% sarcosyl, 5% sucrose.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 23.2 kDa

Predicted isoelectric point: 6.3

[ PREPARATION ]

Reconstitute in PBS, PH7.2-PH7.4 .

[ STORAGE AND STABILITY ]

Stability: The stability of protein is determined by the loss rate of concentration. The loss rate was determined by accelerated thermal degradation test.The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months .

[ SEQUENCES]

The target protein is fused with one N-terminal His tag.

TCVP ASQGLRCIQC ESNQDCLVEE CALGQDLCRT TVLREWEDAE ELEVVTRGCA HKEKTNRTMS

YRMGSVIVSL TETVCATNLC NRPRPGARGR PFPRGRYLEC ASCTSLDQSC ERGREQSLQC RYPTEHCIEV

VTLQSTERSV KDEPYTKGCG SLPGCPGTAG FHSNQTFHFL KCCNFTQCNG GPVLDLQSLP PN

[ REFERENCES ]

1.Rabbani S A., et.al. (1994) FEBS Lett. 338:69-74.

2.The MGC Project Team (2004) Genome Res. 14:2121-2127.

3.Ragno P., et.al. (1992) FEBS Lett. 306:193-198.

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