YB80059Ra01

Parkinson Disease Protein 7 (PARK7)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Parkinson Disease Protein 7 (PARK7)

Gene Names: PARK7

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Protects cells against oxidative stress and cell death.

Subcellular Location: Cytoplasm. Nucleus. Mitochondrion.

Tissue Specificity: Ubiquitous. Detected on epididymal sperm. Highly expressed in testis and prostate. Detected at lower levels in heart, lung, brain, liver, kidney, seminal vesicle, caput and corpus epididymis.

[ PROPERTIES ]

Residues: Met1~Asp189 (Accession # O88767), with a N-terminal His-tag.

Grade & Purity: >97%,  21.49  kDa  as  determined  by  SDS-PAGE  reducing

conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 21.49 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-MASKRALVIL AKGAEEMETV IPVDIMRRAG IKVTVAGLAG KDPVQCSRDV VICPDTSLEE

AKTQGPYDVV VLPGGNLGAQ  NLSESALVKE ILKEQENRKG LIAAICAGPT ALLAHEVGFG CKVTSHPLAK

DKMMNGSHYS YSESRVEKDG LILTSRGPGT SFEFALAIVE ALSGKDMANQ VKAPLVLKD

[ REFERENCES ]

1.Wagenfeld A., et al. (1998) Biochem. Biophys. Res. Commun. 251:545-549.

2.Hod Y., et al. (1999) J. Cell. Biochem. 72:435-444.

 您可以点击 下载产品说明书, 或直接联系我们订购.