YB92285Hu01

Anterior Gradient Protein 2 (AGR2)

Organism: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Anterior Gradient Protein 2

Gene Names: AGR2, AG2

Size: 50µg

Source: Recombinant

Expression Host: E.coli

Function: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells By similarity. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.

Subcellular Location: Secreted. Endoplasmic reticulum.

Tissue Specificity: Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.

[ PROPERTIES ]

Residues: Arg21-Leu175 (Accession # O95994), with N-terminal His-tag.

Grade & Purity: >90%, 23.53 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as solution form in 12mM Sodium phosphate,150mM Sodium chloride，containing 20% glycerol and 300mM imidazole.

Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 23.53 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with two N-terminal tags, His-tag and S-tag, its sequence is listed below.

MH H H H H H S S G LV P R G S G M K E TA A A K F E R Q H M D S P D L G T D D D D K A M A D I G S E F - R D T T V K P G A K

KDTKDSRPKL PQTLSRGWGD QLIWTQTYEE ALYKSKTSNK PLMIIHHLDE CPHSQALKKV FAENKEIQKL

AEQFVLLNLV YETTDKHLSP DGQYVPRIMF VDPSLTVRAD ITGRYSNRLY AYEPADTALL LDNMKKALKL

LKTEL

[ REFERENCES ]

1.Zhang J.-S., et al. (2005) Genes Chromosomes Cancer 43:249-259.

2.Petek, E., et al. (2000) Cytogenet. Cell Genet. 89: 141-142.

3.Thompson D.A., et al. (1998) Biochem. Biophys. Res. Commun. 251:111-116.

4.Wang Z., et al. (2008) Cancer Res. 68:492-497.

5.Park S.-W., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:6950-6955.

6.Fletcher G.C., et al. (2003) Br. J. Cancer 88:579-585.

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