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氢/钾离子交换ATP酶α肽(ATP4α)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradat
The possible reasons that the actual band size differs from the predicted are as follows:
YBG383Hu01 10µg
Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Leu798~Gly896
Tags: N-terminal His-Tag
Accession: P20648
Host: E. coli
Subcellular Location: Membrane; Multi-pass
membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 12.7kDa
Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2.Relative charge: The composition of amino acids may affects the charge of the protein.
3.Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4.Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5.Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
LTP YLI Y ITV SVP LP LG CIT ILFI ELC TDI FPS VS LA YEK AE SD IMH LRP RN PK R DR
LVNEPLAAYS YFQIGAIQSF AGFTDYFTAM AQEGWFPLLC VG
[ REFERENCES ]
1.Maeda M., et al. (1990) J. Biol. Chem. 265:9027-9032.
2.Newman P.R., et al. (1990) DNA Cell Biol. 9:749-762.
3.Grimwood J., et al. (2004) Nature 428:529-535.
4.Sverdlov E.D., et al. (1987) FEBS Lett. 217:275-278.
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