YBG387Ra01 100µg

Recombinant ATPase, H+ Transporting,

Mitochondrial F1 Complex Beta Polypeptide (ATP5b)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Ala47~Ser529

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P10719

Host: E. coli

Subcellular Location: Mitochondrion.

Mitochondrion inner membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 55.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

AA Q S SA A P KA G TAT GQ I VAV I G AV VD V Q F DE G L P PI LN A LE V Q G RE S R LV LE VA

QHLGESTVRT IAMDGTEGLV RGQKVLDSGA PIKIPVGPET LGRIMNVIGE PIDERGPIKT

KQFAPIHAEA PEFIEMSVEQ EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI

NNVAKAHGGY SVFAGVGERT REGNDLYHEM IESGVINLKD ATSKVALVYG QMNEPPGARA

RVALTGLTVA EYFRDQEGQD VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG

TMQERITTTK KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDP

LDSTSRIMDP NIVGSEHYDV ARGVQKILQD YKSLQDIIAI LGMDELSEED KLTVSRARKI

QRFLSQPFQV AEVFTGHMGK LVPLKETIKG FQQILAGDYD HLPEQAFYMV GPIEEAVAKA

DKLAEEHGS

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