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乳酸脱氢酶B(LDHB)重组蛋白
乳酸脱氢酶B(LDHB)重组蛋白

乳酸脱氢酶B(LDHB)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and

Product Introduction


YBB698Mu01 100µg


Recombinant Lactate Dehydrogenase B (LDHB)


Organism Species: Mus musculus (Mouse)


Instruction manual


FOR IN VITRO USE AND RESEARCH USE ONLY


NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES



9th Edition (Revised in Jul, 2013)


[ PROPERTIES ]


Residues: Met1~Leu334 (Accession # P16125), with N-terminal His-Tag.

Host: E. coli


Subcellular Location: Cytoplasm.


Purity: >95%


Endotoxin Level: <1.0EU per 1μg


(determined by the LAL method).


Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.


Predicted isoelectric point: 6.0


Predicted Molecular Mass: 38.1kDa


Applications: SDS-PAGE; WB; ELISA; IP.


(May be suitable for use in other assays to be determined by the end user.)


[ USAGE ]



Reconstitute in sterile PBS, pH7.2-pH7.4.




[ STORAGE AND STABILITY ]


Storage: Avoid repeated freeze/thaw cycles.


Store at 2-8oC for one month.


Aliquot and store at -80oC for 12 months.


Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.


[ SEQUENCES ]


The target protein is fused with N-terminal His-Tag, its sequence is listed below.


MG H H H H H H S G S E F - M AT L K E K L I A SVA D D E A AV P NN K I T V V G V G QV G MA C A I S I


LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR


QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCTIIVVSNP VDILTYVTWK LSGLPKHRVI


G S G C N L D S A R F R Y L M A E K L G I H P S S C H G W I L G E H G D S S VA V W S G V N VA G V


SLQELNPEMG TDNDSENWKE VHKMVVDSAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR


IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLRKS ADTLWDIQKD


LKDL


[ REFERENCES ]


1.Hiraoka B.Y., et al. (1990) Eur. J. Biochem. 189:215-220.


2.Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318.


3.Neidhardt L., et al. (2000) Mech. Dev. 98:77-94.


4.Jovanovic S., et al. (2007) J. Mol. Biol. 371:349-361.

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