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EIF6 Polyclonal Antibody
CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
货号：YBAP11992 |
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规格： 50ul/100ul/200ul |
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价格： 980/1280/1890 |
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| Immunogen： | Recombinant protein of human EIF6 | ||
Synonym: |
CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP) | ||
| Calculated MW: | 27kDa | ||
| Observed MW: | Refer to figures | ||
Source: |
Rabbit | ||
Isotype: |
IgG | Reactivity: H,M,R; Clonality: Polyclonal  |
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Application: |
WB | ||
Purify: |
Affinity purification | ||
Concentration: |
0.95mg/ml | ||
Storage Buffer: |
PBS with 0.05% sodium azide, 50% glycerol, pH7.3 | ||
Storage: |
Store at -20℃. Avoid freeze / thaw cycles. |
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| Western blot analysis of COLO320 cell, using EIF6 Polyclonal Antibody at dilution of 1:500 |
Protocols： |
Buffer-Formulation WB-Guide IHC-Guide IP-Guide IF-Guide |
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Background: |
Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus. In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes. eIF6 is also shown to associate with the RNA-induced silencing complex (RISC). Deletion of eIF6 abolishes the miRNA-mediated gene silencing. eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling.
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