Alternative Names:

GFP ; yfp ; Green fluorescent protein ;

Application:

WB IHC ICC/IF

Reactivity:

All

Dilution:

WB 1:5000~1:10000 IHC 1:100~1:200 ICC/IF 1:100~1:200

	Western blot analysis of GFP expression in 293 cell lysate transfected with GFP.
	Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse liver, using GFP Antibody.
	Immunofluorescent analysis of 293 cells, using GFP Antibody .

Purification:

Affinity-chromatography

Specificity:

GFP antibody detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.

Immunogen:

A synthesized peptide derived from green fluorescent protein

Description:

Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.