Alternative Names:

FLJ21925; ICBP90; MGC138707; Np95; RNF106; hNP95;

Application:

WB

Reactivity:

Human,Mouse

Dilution:

WB 1:1000

	Western blot detection of UHRF1 in Mouse heart,293T,HelaNE and Hela cell lysates using UHRF1 mouse mAb (1:1000 diluted).Predicted band size: 90KDa.Observed band size: 90KDa.

Purification:

Affinity-chromatography

Specificity:

UHRF1(N-term) Mouse mAb detects endogenous levels of total UHRF1(N-term)

Immunogen:

Purified recombinant human UHRF1 protein fragments expressed in E.coil.

Description:

Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.