Alternative Names:

KDM1;AOF2;BHC110;KIAA0601;LSD1; KDM1A;

Application:

WB ICC/IF IP

Reactivity:

Human,Monkey

Dilution:

WB 1:1000 ICC/IF 1:100 IP

	Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-KDM1/LSD1 mouse mAb (dilution 1:100).
	Immunoprecipitation analysis of Hela cell lysates using KDM1/LSD1 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

KDM1/LSD1 Mouse mAb detects endogenous levels of total KDM1/LSD1

Immunogen:

Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.

Description:

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3,thereby acting as a coactivator or a corepressor,depending on the context.Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.Acts as a corepressor by mediating demethylation of H3K4me,a specific tag for epigenetic transcriptional activation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.