Alternative Names:

Apoptotic cell clearance receptor; JmjC domain-containing protein 6; JMJD6; Protein PTDSR; PSR; PTDSR;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:200

	Western blot detection of JMJD6(N-terminus) in Hela and HL-60 lysates using JMJD6(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 46KDa.Observed band size: 62KDa.
	Immunofluorescent analysis of HeLa cells using anti-JMJD6(N-terminus) mouse mAb diluted 1:200.

Purification:

Affinity-chromatography

Specificity:

JMJD6(N-term) Mouse mAb detects endogenous levels of total JMJD6(N-term)

Immunogen:

Purified recombinant human JMJD6(N-terminus) fragments expressed in E.coli.

Description:

Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.