Alternative Names:

EG5; HKSP; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; KNSL1; MCLMR; TR-interacting protein 5; TRIP5;

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:200 IP

	Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.
	Immunofluorescent analysis of Hela using Eg5 mouse mAb (1:200).
	Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Eg5 Mouse mAb detects endogenous levels of total Eg5

Immunogen:

Purified recombinant human Eg5 protein fragments expressed in E.coli.

Description:

Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.