Alternative Names:

SOX2; ANOP3; MCOPS3; MGC2413; lcc; SRY related HMG box 2;

Application:

WB ICC/IF FC

Reactivity:

Mouse

Dilution:

WB 1:1000 ICC/IF 1:150 FC 1:100

	Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.
	Immunofluorescent analysis of COS7 cells using anti-Sox2 mouse mAb diluted 1:150.
	Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

Purification:

Affinity-chromatography

Specificity:

Sox2 Mouse mAb detects endogenous levels of total Sox2

Immunogen:

Purified recombinant mouse Sox2 protein fragments expressed in E.coli

Description:

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206 by similarity.Critical for early embryogenesis and for embryonic stem cell pluripotency.May function as a switch in neuronal development.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.