Alternative Names:

PP-1A; PPP1A; MGC1674; MGC15877; PP1alpha; PPP1CA;

Application:

WB IHC ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000 IHC 1:500 ICC/IF 1:300

	Western blot detection of PPP1A in MCF7,K562,Hela,HEPG2 and Jurkat whole cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa.
	IHC of paraffin-embedded human breast cancer using anti-PPP1A mouse mAb diluted 1/500-1/1000.
	Immunofluorescent analysis stain of Hela using PPP1A mouse mAb (1:300).

Purification:

Affinity-chromatography

Specificity:

PPP1A Mouse mAb detects endogenous levels of total PPP1A

Immunogen:

Purified recombinant human PPP1A protein fragments expressed in E.coli.

Description:

Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.