Alternative Names:

PP4; PPX; PP4C; PPH3; PPP4;

Application:

WB IHC ICC/IF

Reactivity:

Human

Dilution:

WB 1:200 IHC 1:500 ICC/IF 1:200

	Western blot detection of Protein Phosphatase 4C in Hela and Jurkat cell lysates using Protein Phosphatase 4C mouse mAb (1:200 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
	IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C mouse mAb diluted 1/500-1/1000.
	Immunofluorescent analysis of HeLa cells using anti-Protein Phosphatase 4C mouse mAb diluted 1:200.

Purification:

Affinity-chromatography

Specificity:

Protein Phosphatase 4C Mouse mAb detects endogenous levels of total Protein Phosphatase 4C

Immunogen:

Purified recombinant human Protein Phosphatase 4C protein fragments expressed in E.coli.

Description:

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.