Alternative Names:

Antigen NY-CO-13; TP53; Tumor suppressor p53; Cellular tumor antigen p53; Phosphoprotein p53;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:1000

	Western blot detection of p53 in SW480,T47D and MDA-MB-468 cell lysates using p53 mouse mAb (1:1000 diluted).Predicted band size: 53KDa.Observed band size: 53KDa.

Purification:

Affinity-chromatography

Specificity:

p53(C-term) Mouse mAb detects endogenous levels of total p53(C-term)

Immunogen:

Purified recombinant human p53 protein fragments expressed in E.coli.

Description:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.