Alternative Names:

Chromosome segregation protein SmcB; DXS423E; KIAA0178; SB1.8/DXS423E protein; SM1A; SMC1A; SMC1L1; SMC1alpha protein; Structural maintenance of chromosome 1-like 1 protein;

Application:

WB IHC FC

Reactivity:

Human

Dilution:

WB 1:1000 IHC 1:500 FC 1:100

	Western blot detection of SMC1A(N-terminus) in HelaNE,Jurkat and A431 cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.
	IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) mouse mAb diluted 1/500-1/1000.
	Flow Cytometry analysis of HeLa cells stained with SMC1A (N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

Purification:

Affinity-chromatography

Specificity:

SMC1A(N-term) Mouse mAb detects endogenous levels of total SMC1A(N-term)

Immunogen:

Purified recombinant human SMC1A(N-terminus) protein fragments expressed in E.coli.

Description:

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.