Alternative Names:

Pyruvate dehydrogenase E1 component subunit alpha, somatic form; mitochondrial; PDHE1-A type I; PDHA1; PHE1A;

Application:

WB ICC/IF

Reactivity:

Human,Mouse

Dilution:

WB 1:1000 ICC/IF 1:100

	Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293,1299,mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb(dilution 1:100).

Purification:

Affinity-chromatography

Specificity:

pyruvate dehydrogenase (lipoamide) α 1 Mouse mAb detects endogenous levels of total pyruvate dehydrogenase (lipoamide) α 1

Immunogen:

Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.

Description:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.