Alternative Names:

ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta;

Application:

WB IP

Reactivity:

Human

Dilution:

WB 1:1000 IP

	Western blot detection of SBRG1 in Hela NE and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.
	Immunoprecipitation analysis of Hela cells lysates. IP: BRG1 mouse mAb(200282); WB: BRG1 mouse mAb(201025).

Purification:

Affinity-chromatography

Specificity:

BRG1 Mouse mAb detects endogenous levels of total BRG1

Immunogen:

Purified recombinant human BRG1 protein fragments expressed in E.coli.

Description:

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation.Component of the CREST-BRG1 complex,a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex.In resting neurons,transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.