Alternative Names:

TRIM33; Ectodermin homolog; ECTO; RET-FUSED GENE 7; RFG7; TF1G; TIF1-gamma; PTC7; TIF1G; Tripartite motif-containing 33; Protein Rfg7; Tripartite motif containing 33

Application:

WB

Reactivity:

Human

Dilution:

WB 1:1000

	Western blot detection of TIF1 gamma / TRIM33 in MCF7 and HelaNE cell lysates using TIF1 gamma / TRIM33 mouse mAb (1:1000 diluted).Predicted band size: 120KDa.Observed band size: 140KDa.

Purification:

Affinity-chromatography

Specificity:

TIF1γ/TRIM33 Mouse mAb detects endogenous levels of total TIF1γ/TRIM33

Immunogen:

Purified recombinant human TIF1 gamma / TRIM33 protein fragments expressed in E.coli.

Description:

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.