Alternative Names:

GRP-78; GRP78; BIP; MIF2; FLJ26106; HSPA5

Application:

WB ICC/IF IP

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000 ICC/IF 1:200 IP

	Western blot analysis of extracts from 3T3,A549,C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.
	Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20℃ and using BiP/GRP78 mouse mAb (dilution 1:200).
	Immunoprecipitation of BiP/GRP78 from Hela cell extracts using BiP/GRP78 Mouse mAb.Western blot was performed using BiP/GRP78 Mouse mAb.

Purification:

Affinity-chromatography

Specificity:

BiP/GRP78 Mouse mAb detects endogenous levels of total BiP/GRP78

Immunogen:

Purified recombinant human BiP/GRP78 protein expressed in E.coli.

Description:

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.