Alternative Names:

GRP-78; GRP78; BIP; MIF2; FLJ26106; HSPA5

Application:

WB IHC ICC/IF

Reactivity:

Human,Rat

Dilution:

WB 1:1000 IHC 1:100 ICC/IF 1:50

	Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.
	Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).

Purification:

Affinity-chromatography

Specificity:

BiP/GRP78(C-term) Mouse mAb detects endogenous levels of total BiP/GRP78(C-term)

Immunogen:

Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.

Description:

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.