Alternative Names:

ATP dependent helicase CHD3; CHD-3; Chd3; Chd7; Chromodomain-helicase-DNA-binding protein 3; hZFH; Mi 2 autoantigen 240 kDa protein; Mi 2a; Mi2 ALPHA; Prp7; ZFH; Zinc finger helicase;

Application:

WB IHC FC

Reactivity:

Human

Dilution:

WB 1:500-1000 IHC 1:500 FC 1:100

	Western blot detection of CHD3 (C-terminus) in Y79 and Raji cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 226KDa.Observed band size: 260KDa.
	IHC of paraffin-embedded human colon using anti-CHD3 (C-terminus) diluted 1/500-1/1000.
	Flow Cytometry analysis of K562 cells stained with CHD3 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

Purification:

Affinity-chromatography

Specificity:

CHD3(C-term) Mouse mAb detects endogenous levels of total CHD3(C-term)

Immunogen:

Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.

Description:

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.