Alternative Names:

C21; DC42; F box like/WD repeat containing protein TBL1XR1; IRA1; Nuclear receptor corepressor/HDAC3 complex subunit; TBL1 related protein 1;

Application:

WB IHC ICC/IF

Reactivity:

Human,Mouse

Dilution:

WB 1:500-1000 IHC 1:500 ICC/IF 1:200

	Western blot detection of TBLR1 in Mouse brain,Mouse heart and K562 cell lysates using TBLR1 mouse mAb (1:1000 diluted).Predicted band size: 60KDa.Observed band size: 60Kda.
	IHC of paraffin-embedded huma breast cancer using anti-TBLR1 mouse mAb diluted 1/500-1/1000
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using TBLR1 mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

TBLR1 Mouse mAb detects endogenous levels of total TBLR1

Immunogen:

Purified recombinant human TBLR1 protein fragments expressed in E.coli.

Description:

F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.