Alternative Names:

PKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3;

Application:

WB

Reactivity:

Mouse,Rat

Dilution:

WB 1:1000

	Western blot detection of total AKT in rat heart,rat testis,mouse brain,mouse spleen and C6 cell lysates and using AKT(pan) mouse mAb (1:1000 diluted).Predicted band size: 60KDa.Observed band size: 60KDa.

Purification:

Affinity-chromatography

Specificity:

Akt(pan) Mouse mAb detects endogenous levels of total Akt(pan)

Immunogen:

Purified recombinant human AKT1 protein fragments expressed in E.coli.

Description:

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.