Alternative Names:

Dwfc; hSmad5; JV5-1 ; MADH5; SMAD5; MusMLP ;SMAD family member 5;

Application:

WB ICC/IF FC

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:75 FC 1:100

	Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.
	Immunofluorescent analysis of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
	Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

Purification:

Affinity-chromatography

Specificity:

SMAD5(C-term) Mouse mAb detects endogenous levels of total SMAD5(C-term)

Immunogen:

Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.

Description:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.