Alternative Names:

ACL; ATPCL; CLATP; ATP citrate lyase;

Application:

WB ICC/IF FC

Reactivity:

Human,Mouse,Monkey

Dilution:

WB 1:1000 ICC/IF 1:150 FC 1:100

	Western blot detection of ATP-Citrate Lyase in 3T3,K562,COS7 and Hela cell lysates using ATP-Citrate Lyase mouse mAb (1:1000 diluted).Predicted band size: 120KDa.Observed band size: 120KDa.
	Immunofluorescent analysis of HeLa cells using anti-ATP-Citrate Lyase (C-terminus) mouse mAb diluted 1:150.
	Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG

Purification:

Affinity-chromatography

Specificity:

ATP-Citrate Lyase(C-term) Mouse mAb detects endogenous levels of total ATP-Citrate Lyase(C-term)

Immunogen:

Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.

Description:

ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.