Alternative Names:

TFIIH basal transcription factor complex p62 subunit; BTF2-p62; General transcription factor IIH polypeptide 1; GTF2H1; BTF2;

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:300 ICC/IF 1:50 IP

	Western blot detection of GTF2H1 in Hela,Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).
	Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

GTF2H1 Mouse mAb detects endogenous levels of total GTF2H1

Immunogen:

Purified recombinant human GTF2H1protein fragments expressed in E.coli.

Description:

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.