Alternative Names:

SIRT2;SIR2;SIR2L;SIR2L2;

Application:

WB ICC/IF

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000 ICC/IF 1:50

	Western blot detection of SirT2 in PC-12, Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39,43KDa. Observed band size:39,43KDa.
	Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20℃ and using SirT2 (200474,dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10) (310045,dilution 1:200) Rabbit pAb (red).

Purification:

Affinity-chromatography

Specificity:

SirT2 Mouse mAb detects endogenous levels of total SirT2

Immunogen:

Purified recombinant human SirT2 protein expressed in E.coli.

Description:

Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. Plays a major role in the control of cell cycle progression and genomic stability. Functions in the antephase checkpoint preventing precocious mitotic entry in response to microtubule stress agents, and hence allowing proper inheritance of chromosomes.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.