Alternative Names:

Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18; Keratin-18; CYK18; cytokeratin 18; Cytokeratin-18; K18; K1C18; keratin 18; KRT18; cell proliferation-inducing protein 46; CK-18;

Application:

WB ICC/IF

Reactivity:

Human,Monkey

Dilution:

WB 1:2000 ICC/IF 1:800

	Western blot detection of Keratin 18 in Hela,COS7 and 293T cell lysates using Keratin 18 mouse mAb (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
	Immunofluorescent analysis of HeLa cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-Keratin 18 mouse mAb (dilution 1:800).

Purification:

Affinity-chromatography

Specificity:

Keratin 18 Mouse mAb detects endogenous levels of total Keratin 18

Immunogen:

Purified recombinant human Keratin 18 protein fragments expressed in E.coli.

Description:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.