Alternative Names:

Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3beta isoform;

Application:

WB ICC/IF IP

Reactivity:

Human,Mouse

Dilution:

WB 1:1000 ICC/IF 1:200 IP

	Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3β mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
	Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using GSK-3β mouse mAb (dilution 1:200).
	Immunoprecipitation analysis of Hela cell lysates using GSK-3β.GSK-3β Rabbit mAb was used for the western blot analysis (1:1000 diluted).

Purification:

Affinity-chromatography

Specificity:

GSK3 β Mouse mAb detects endogenous levels of total GSK3 β

Immunogen:

Purified recombinant GSK-3β protein fragments expressed in E.coli.

Description:

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.