Alternative Names:

CCND 2; CCND2; CyclinD2; G1/S specific cyclin D2; G1/S-specific cyclin-D2;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:1000

	Western blot detection of Cyclin D2 in CHO-K1 cell lysate（A）and CHO-K1 transfected by Cyclin D2-fragment EGFP fusion protein（B）cell lysate using Cyclin D2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Purification:

Affinity-chromatography

Specificity:

Cyclin D2 Mouse mAb detects endogenous levels of total Cyclin D2

Immunogen:

Purified recombinant human Cyclin D2 protein fragments expressed in E.coli.

Description:

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition.Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.