Alternative Names:

IK1; LYF1; hIk-1; IKAROS; PRO0758; ZNFN1A1; Hs.54452;

Application:

WB IP

Reactivity:

Human

Dilution:

WB 1:1000 IP

	Western blot detection of Ikaros(C-terminus) in Ramos and Jurkat cell lysates using Ikaros(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 58KDa.
	Immunoprecipitation analysis of Hela cell lysate using Ikaros (C-terminus) mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Ikaros(C-term) Mouse mAb detects endogenous levels of total Ikaros(C-term)

Immunogen:

Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.

Description:

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.