Alternative Names:

Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; PSKJ3; CDK4; CMM 3;

Application:

WB

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000

	Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

Purification:

Affinity-chromatography

Specificity:

CDK4 Mouse mAb detects endogenous levels of total CDK4

Immunogen:

Purified recombinant human CDK4 protein fragments expressed in E.coli.

Description:

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.