Alternative Names:

CREB regulated transcription coactivator 1; CRTC1; MECT 1;Mucoepidermoid carcinoma translocated 1; TORC1;Transducer of CREB protein 1;

Application:

WB ICC/IF IP FC

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:300 IP FC 1:100

	Western blot detection of MECT1 / Torc1 in Hela and Jurkat lysates using MECT1 / Torc1 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 78KDa.
	Immunofluorescent analysis stain of Hela using MECT1 / Torc1 mouse mAb (1:300).
	Immunoprecipitation analysis of Hela cell lysate using MECT1 / Torc1 mouse mAb.
	Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

Purification:

Affinity-chromatography

Specificity:

MECT1/Torc1 Mouse mAb detects endogenous levels of total MECT1/Torc1

Immunogen:

Purified recombinant human MECT1 / Torc1 protein fragments expressed in E.coli.

Description:

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.