Alternative Names:

CAK; CCNH; CDK-activating kinase; cyclin H; cyclin-dependent kinase-activating kinase; Cyclin-H; MO15-associated protein; p34; p37;

Application:

WB IP

Reactivity:

Human

Dilution:

WB 1:1000 IP

	Western blot detection of Cyclin H in Jurkat,K562 and A431 cell lysates using Cyclin H mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa.
	Immunoprecipitation analysis of Hela cell lysates using Cyclin H mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Cyclin H Mouse mAb detects endogenous levels of total Cyclin H

Immunogen:

Purified recombinant human Cyclin H protein fragments expressed in E.coli.

Description:

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.