Alternative Names:

CDK5; CRK6; Cell division protein kinase 5; EC 2.7.11.22; PSSALRE; TAU protein kinase II catalytic subunit; TPKII catalytic subunit; Tau protein kinase II catalytic subunit; kinase Cdk5;

Application:

WB ICC/IF

Reactivity:

Human,Mouse,Rat,Monkey

Dilution:

WB 1:500 ICC/IF 1:150

	Western blot detection of CDK5(N-terminus) in 3T3,Hela,PC-12,COS7,Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.
	Immunofluorescent analysis of HeLa cells using anti-CDK5(N-terminus) mouse mAb diluted 1:150

Purification:

Affinity-chromatography

Specificity:

CDK5(N-term) Mouse mAb detects endogenous levels of total CDK5(N-term)

Immunogen:

Purified recombinant human CDK5(N-terminus) protein fragments expressed in E.coli.

Description:

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.