Alternative Names:

CDC2; DKFZp686L20222; CDC28A; P34CDC2; MGC111195; CDK1

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:100 ICC/IF 1:50

	Western blot detection of CDC2/CDK1 in K562,A549,Jurkat and Hela cell lysates using CDC2/CDK1 mouse mAb (1:100 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
	Immunofluorescent analysis of HeLa cells using anti-CDC2/CDK1 mouse mAb diluted 1:50.

Purification:

Affinity-chromatography

Specificity:

CDC2/CDK1 Mouse mAb detects endogenous levels of total CDC2/CDK1

Immunogen:

Purified recombinant human CDC2/CDK1 protein fragments expressed in E.coli.

Description:

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.