Alternative Names:

EEF 2; Eef2; EF-2; EF2; Elongation factor 2; Polypeptidyl tRNA translocase; SCA26;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:5000 ICC/IF 1:200

	Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.
	Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using anti-eEF2 mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

eEF2 Mouse mAb detects endogenous levels of total eEF2

Immunogen:

Purified recombinant human eEF2 protein fragments expressed in E.coli.

Description:

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.