Alternative Names:

HAP; HET;HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; SAF-B1; SAFB1; scaffold attachment factor B1;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:200

	Western blot detection of SAFB1 in HelaNE,A431,MDA-MB-468,Breast cancer,ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

SAFB1 Mouse mAb detects endogenous levels of total SAFB1

Immunogen:

Purified recombinant human SAFB1 protein fragments expressed in E.coli.

Description:

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.