Alternative Names:

Tyrosine-protein kinase CSK; C-SRC kinase; Protein-tyrosine kinase CYL; CSK;

Application:

WB ICC/IF

Reactivity:

Human,Rat

Dilution:

WB 1:1000 ICC/IF 1:50

	Western blot detection of CSK in C6 and Jurkat cell lysates using CSK mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
	Immunofluorescent analysis of C6 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-CSK mouse mAb (dilution 1:50).

Purification:

Affinity-chromatography

Specificity:

CSK Mouse mAb detects endogenous levels of total CSK

Immunogen:

Purified recombinant human CSK protein fragments expressed in E.coli.

Description:

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.