Alternative Names:

DNA- PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; P460; PRKD; PRKDC; XRCC7; kinase DNA-PK;

Application:

WB IHC ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:1000 IHC 1:200 ICC/IF 1:100 IP

	Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.
	Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
	Immunofluorescent analysis stain of Hela using DNA-PKcs mouse mAb (1:100).
	Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.

Purification:

Affinity-chromatography

Specificity:

DNA-PKcs Mouse mAb detects endogenous levels of total DNA-PKcs

Immunogen:

Purified recombinant human DNA-PKcs protein fragments expressed in E.coli

Description:

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage.Involved in DNA nonhomologous end joining(NHEJ)required for double-strand break(DSB)repair and V(D)J recombination.Must be bound to DNA to express its catalytic properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.