Alternative Names:

DLAT; DLAT; DLTA M2 antigen complex 70 kDa subunit; M2 Antigen Complex 70kD Subunit; PBC; PDC E2; PDC-E2; PDCE2;

Application:

WB ICC/IF IP

Reactivity:

Human,Mouse

Dilution:

WB 1:1000 ICC/IF 1:300 IP

	Western blot detection of Pyruvate Dehydrogenase E2 in Jurkat,A549,U251,F9,Lncap and Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb (1:1000 diluted).Predicted band size:69KDa.Observed band size:69KDa.
	Immunofluorescent analysis stain of Hela using Pyruvate Dehydrogenase E2 mouse mAb (1:300).
	Immunoprecipitation analysis of Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Pyruvate Dehydrogenase E2 Mouse mAb detects endogenous levels of total Pyruvate Dehydrogenase E2

Immunogen:

Purified recombinant human Pyruvate Dehydrogenase E2 protein fragments expressed in E.coli.

Description:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.