Alternative Names:

CPT7; CYP17; P450C17; S17AH; CYP17A1;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:1000

	Western blot detection of Cytochrome P450 17A1 in Hela,HepG2 and A549 cell lysates using Cytochrome P450 17A1 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Purification:

Affinity-chromatography

Specificity:

Cytochrome P450 17A1 Mouse mAb detects endogenous levels of total Cytochrome P450 17A1

Immunogen:

Purified recombinant human Cytochrome P450 17A1 protein fragments expressed in E.coli.

Description:

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione.Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.Involved in sexual development during fetal life and at puberty.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.