Alternative Names:

Adapter molecule crk; CRK; CRKII;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:1000

	Western blot detection of CrkII in Lncap,A431,MCF7 and HT1080 cell lysates using CrkII mouse mAb (1:1000 diluted).Predicted band size: 34kDa.Observed band size: 34kDa.

Purification:

Affinity-chromatography

Specificity:

CrkII Mouse mAb detects endogenous levels of total CrkII

Immunogen:

Purified recombinant human CrkII protein fragments expressed in E.coli.

Description:

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.