Alternative Names:

Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;

Application:

WB ICC/IF

Reactivity:

Human,Monkey

Dilution:

WB 1:1000 ICC/IF 1:300

	Western blot detection of UCHL1 / PGP9.5 in U251,IMR32,Raji and COS7 cell lysates and using UCHL1 / PGP9.5 mouse mAb (1:1000 diluted).Predicted band size: 25KDa.Observed band size: 28KDa.
	Immunofluorescent analysis stain of COS7 using UCHL1 / PGP9.5 mouse mAb (1:300).

Purification:

Affinity-chromatography

Specificity:

UCHL1/PGP9.5 Mouse mAb detects endogenous levels of total UCHL1/PGP9.5

Immunogen:

Purified recombinant human UCHL1 / PGP9.5 protein fragments expressed in E.coli.

Description:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.