Alternative Names:

28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27;

Application:

WB ICC/IF

Reactivity:

Human,Monkey

Dilution:

WB 1:1000 ICC/IF 1:300

	Western blot detection of Hsp27 in Hela,HsHela,A431,COS7,Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted).Predicted band size: 23KDa.Observed band size:27KDa.
	Immunofluorescent analysis stain of Hela using Hsp27 mouse mAb (1:300).

Purification:

Affinity-chromatography

Specificity:

Hsp27 Mouse mAb detects endogenous levels of total Hsp27

Immunogen:

Purified recombinant human Hsp27 protein fragments expressed in E.coli.

Description:

Involved in stress resistance and actin organization.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.