Alternative Names:

JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2; Mothers against decapentaplegic homolog 2; Smad 2;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:500 ICC/IF 1:100

	Western blot detection of Smad2 in Hela,A431,Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.
	Immunofluorescent analysis of HeLa cells fixed with 1% Paraformaldehyde and using Smad2 mouse mAb (dilution 1:100).

Purification:

Affinity-chromatography

Specificity:

Smad2 Mouse mAb detects endogenous levels of total Smad2

Immunogen:

Purified recombinant human Smad2 protein fragments expressed in E.coli.

Description:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and,on formation of the SMAD2/SMAD4 complex,activates transcription.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.